產品編號 yb-0059R
英文名稱 Anti-APAF1(NT)抗體
中文名稱 凋亡蛋白活性因子-1抗體（N端）
別    名 APAF 1; APAF1; APAF-1; Apoptotic peptidase activating factor 1; Apoptotic protease activating factor; CED 4; CED4; KIAA0413; APAF_HUMAN; Apoptotic protease-activating factor 1.
 Anti-APAF1(NT)抗體 
說 明 書 0.1ml  0.2ml  
研究領域 腫瘤  細胞生物  神經生物學  信號轉導  細胞凋亡  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse, Rat, Chicken, Dog, Cow, Horse, 
產品應用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 137kDa
細胞定位 細胞漿 
性    狀 Lyophilized or Liquid
濃    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human Apaf-1
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
 Anti-APAF1(NT)抗體
PubMed PubMed
產品介紹 background:
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain(NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].

Function:
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.

Subunit:
Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain).

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.

Similarity:
Contains 1 CARD domain. 
Contains 1 NB-ARC domain. 
Contains 13 WD repeats.

 Anti-APAF1(NT)抗體調節*依賴的Caspase-9原的自動催化活性，導致Caspase-3 的激活和引起凋亡。
Apaf-1在成年人的脾臟、外周血白細胞、腎臟、肺和胎兒腦、腎、肺中高水平表達。